Rhona Mitra appeared in the final season of The Practice as Tara Wilson, and continued that role into its spin-off Boston Legal, but left not long into the second season. In 2005, Mitra played the role of Kit McGraw during Season 3 of Nip/Tuck. Mitra then went on to appear in Skinwalkers, The Number 23 and Shooter. In 2008, Rhona Mitra starred in the lead role of the science fiction/action film Doomsday as Major Eden Sinclair, and in 2009 went on to star in Underworld: Rise of the Lycans as Sonja, the daughter of the powerful vampire elder Viktor (played by Bill Nighy) and is currently appearing in Stargate Universe.
Rhona Mitra appeared as the live action model for Lara Croft, the lead character in Eidos Interactive's Tomb Raider video game series. Rhona Mitra was ranked #46 on the Maxim Hot 100 Women of 2001. Rhona Mitra stars in the 2010 Anders Anderson thriller film Stolen, alongside Josh Lucas and Jon Hamm. Rhona Mitra portrays Claire Radcliff in the ABC Science-Fiction series The Gates. 